PNABIO INC公司产品介绍【代理商代购购买现货】

PNABIOINC公司产品介绍【代理商代购购买现货】

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4000-520-616

Pnabio公司致力于为基础研究和应用领域的实验室提供高质量的研究产品和服务。

PNABio提供优质的PNA(肽核酸)产品,包括定制的寡糖,鱼探针,miRNA抑制剂,PNA钳探针和Fmoc单体。HPLC纯化后提供>95%的纯度,并附有MS/MS数据。PNA产品由Panagene公司生产。

PNABio还提供工程核酸酶服务,包括CRISPR/Cas9核酸酶(RGEN)合成、验证和Cas9蛋白以及敲除和敲入细胞系服务。RGEN及相关产品由


PNAClamp技术是基于PNA的PCR钳位技术,可以选择性扩增突变的目标DNA序列,作为与主要野生型DNA序列混合的次要部分。它充分利用PNA探针的性质,对其靶DNA具有强结合亲和力和特异性,而不被DNA聚合酶识别为引物

PNAClamp突变检测试剂盒可以使用来自手术组织或福尔马林固定石蜡包埋的活组织检查(FFPE)的DNA样本。

PNAClamp突变检测试剂盒与传统方法相比具有许多优点。

在短时间内检测到突变(3小时运行时间,30分钟动手时间)
简单的协议
检测需要少量的DNA样品(1〜25ng)
高灵敏度(检测限<1%)
一贯
保质期长(>1年)
与各种实时PCR仪(ABI-7500,ABI-7900HT,BioradCFX-96/384,RocheLightcycler,QiagenRGQ等)兼容。
用于简单数据解释的分析软件
我们还提供专门的来建立和分析数据。


部分产品:

WeprovidecustomCRISPR/Cas9(RGEN)synthesisservicewithorwithoutvalidation.

IVTsgRNASynthesisService(aRGEN)

  • Includesdesign,off-targetsearch,andsynthesis

  • Provides50ugsgRNA,lyophilizedform

  • Injection/transfectionready

  • Quality/quantityconfirmedinagarosegel

  • Timeline:2weeks


sgRNAVectorSynthesisService(dRGEN)

  • Includesdesign,off-targetsearch,andsynthesis

  • ProvidesanexpressionplasmidofsgRNAandCas9

  • Timeline:2weeks

  • Cas9vectorsavailABLewithcoexpressionofGFP/HygroRandRFP/PuroRcassetteunderCMVorEF1apromoterforselection

sgRNAValidationService

  • Customerprovidesgenename,species,andprojectgoal(KO,KIetc)

  • Includesdesign,off-targetsearch,andsynthesisof4~8gRNAvectors

  • TransfectCRISPR/Cas9intocellsandperformmismatchsensitivenucleaseassaytofindthemostefficientnuclease

  • sgRNAisprovidedinanexpressionplasmid(dRGEN)orRNAformat(aRGEN)

  • PCRprimers,PCRcondition,andmismatchsensitivenucleaseassayforKOscreeningisalsoprovided

  • Timeline:3~6weeks

Cas9vectors

  • CV01&CV11:Cas9(CMVorEF1apromoter)

  • CV02&CV12:Cas9-HygR-GFP(CMVorEF1apromoter)

  • CV03&CV13:Cas9-RFP-PuroR(CMVorEF1apromoter)

GuideRNA(gRNA)Design

Ingeneral,findgRNAsequencewithnooff-targetsfor1bpand2bpmismatches,andcontains45~70%GC.Ifyoucannotavoidoff-targets,mismatcheslocatednearPAMarepreferredovertheonesat5'sideofgRNA.Pleasenotethatnon-canonicalPAM(-NAG,-NGA)canalsobecleaved.
Wehavedevelopedour gRNATool thatoutputsoff-targetsequences,GCcontentandotherfeatures.Youcanalsousepublicallyavailabletoolstosearchwithvariousoptions.
Cas-Designer
ChopChop
E-CRISP
Cas-Offinder (toseetheoff-targetlist)

eGFPControlsgRNA

  • ValidatedforcuttingeGFPbothinvitroandinvivo

  • Injectionreadyform,lyophilizedpowder

  • AlsoavailablewitheGFPplasmidandCas9proteinaspositivecontrolreagentsforinvitrocleavageassay

  • Availablesizes:20ugor100ug(20ugx5tubes)

  • Catalognumber:AR04

SurrogateReporter

  • Tovalidatetheactivityofvariousnucleases(CRISPR/Cas9,TALEN,andZFN)incells

  • Alsotoenrichcellswithgeneeditingeventsforclonescreening

  • UseFACS,MACSordrugselection

  • Choiceofemptyplasmidorcustomdesignedforthenucleasetargetsequence

  • Pleasereferto"ReportersforNucleases"formoredetails

  • Timeline:2weeksforcustomsurrogatereportervectors


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